Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5aPKC Signaling Pathway
Joint Authors
He, Jinxi
Yang, Jiali
Shi, Juan
Xue, Jing
Liu, Xiaoming
Chen, Juan
Zhang, Kangjian
Shi, Juan
Li, Jing
Zhu, Yongzhao
Wei, Jun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-08
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The development of chemoresistance to cisplatin regimens causes a poor prognosis in patients with advanced NSCLC.
The role of noncanonical Wnt signaling in the regulation of properties of lung cancer stem cells and chemoresistance was interrogated, by accessing capacities of cell proliferation, migration, invasion, and clonogenicity as well as the apoptosis in A549 cell lines and cisplatin-resistant A549 cells treated with Wnt5a conditional medium or protein kinase C (PKC) inhibitor GF109203X.
Results showed that the noncanonical Wnt signaling ligand, Wnt5a, could promote the proliferation, migration, invasion, and colony formation in A549 lung adenocarcinoma cells and cisplatin-resistant A549/DDP cells and increase the fraction of ALDH-positive cell in A549/DDP cells.
An exposure of cells to Wnt5a led to a significant reduction of A549/DDP cell apoptosis but not A549 cells.
An addition of GF109203X could both strikingly increase the baseline apoptosis and resensitize the Wnt5a-inhibited cell apoptosis.
Interestingly, an inhibition of Wnt/PKC signaling pathway could reduce properties of lung cancer stem cells, promote cell apoptosis, and resensitize cisplatin-resistant cells to cisplatin via a caspase/AIF-dependent pathway.
These data thus suggested that the Wnt5a could promote lung cancer cell mobility and cisplatin-resistance through a Wnt/PKC signaling pathway and a blockage of this signaling may be an alternative therapeutic strategy for NSCLC patients with resistance to chemotherapies.
American Psychological Association (APA)
Yang, Jiali& Zhang, Kangjian& Shi, Juan& Shi, Juan& Xue, Jing& Li, Jing…[et al.]. 2016. Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5aPKC Signaling Pathway. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116101
Modern Language Association (MLA)
Yang, Jiali…[et al.]. Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5aPKC Signaling Pathway. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1116101
American Medical Association (AMA)
Yang, Jiali& Zhang, Kangjian& Shi, Juan& Shi, Juan& Xue, Jing& Li, Jing…[et al.]. Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5aPKC Signaling Pathway. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116101