Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study

Joint Authors

Cui, Li-li
Kinnunen, Tuure
Boltze, Johannes
Nystedt, Johanna
Jolkkonen, Jukka

Source

Stem Cells International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-02-28

Country of Publication

Egypt

No. of Pages

8

Abstract EN

Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells.

Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk.

We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2–2.0 × 106/mL), storage solutions (complete growth medium, Dulbecco’s phosphate-buffered saline, and normal saline), storage time in suspension (0–9 h), and freeze-thawing procedure on the clumping of rat bone marrow derived mesenchymal stromal cells (BMMSCs) and also evaluated cell viability at the same time.

Surprisingly, increasing the cell concentration did not result in more cell clumps in vitro.

Freshly harvested (fresh) cells in normal saline had significantly fewer cell clumps and also displayed high viability (>90%).

A time-dependent reduction in viability was observed for cells in all three storage solutions, without any significant change in the clumping tendency except for cells in medium.

Fresh cells were more viable than their frozen-thawed counterparts, and fresh cells in normal saline had fewer cell clumps.

In conclusion, cell clumping and viability could be affected by different cell preparation procedures, and quantification of cell clumping can be conducted using the flow cytometry-based pulse-width assay before intra-arterial cell delivery.

American Psychological Association (APA)

Cui, Li-li& Kinnunen, Tuure& Boltze, Johannes& Nystedt, Johanna& Jolkkonen, Jukka. 2016. Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study. Stem Cells International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116127

Modern Language Association (MLA)

Cui, Li-li…[et al.]. Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1116127

American Medical Association (AMA)

Cui, Li-li& Kinnunen, Tuure& Boltze, Johannes& Nystedt, Johanna& Jolkkonen, Jukka. Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116127

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1116127