Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
Joint Authors
Ai, Zhiying
Shao, Jingjing
Shi, Xinglong
Yu, Mengying
Wu, Yongyan
Du, Juan
Zhang, Yong
Guo, Zekun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-07
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications.
It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs.
However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK.
Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation.
Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development.
Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.
American Psychological Association (APA)
Ai, Zhiying& Shao, Jingjing& Shi, Xinglong& Yu, Mengying& Wu, Yongyan& Du, Juan…[et al.]. 2015. Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116132
Modern Language Association (MLA)
Ai, Zhiying…[et al.]. Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116132
American Medical Association (AMA)
Ai, Zhiying& Shao, Jingjing& Shi, Xinglong& Yu, Mengying& Wu, Yongyan& Du, Juan…[et al.]. Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116132