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Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells
Joint Authors
Marino, Victor
Xiong, Jimin
Menicanin, Danijela
Zilm, Peter S.
Bartold, P. Mark
Gronthos, Stan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-04
Country of Publication
Egypt
No. of Pages
13
Abstract EN
The present study examined the cell surface proteome of human periodontal ligament stem cells (PDLSC) compared to human fibroblasts.
Cell surface proteins were prelabelled with CyDye before processing to extract the membrane lysates, which were separated using 2D electrophoresis.
Selected differentially expressed protein “spots” were identified using Mass spectrometry.
Four proteins were selected for validation: CD73, CD90, Annexin A2, and sphingosine kinase 1 previously associated with mesenchymal stem cells.
Flow cytometric analysis found that CD73 and CD90 were highly expressed by human PDLSC and gingival fibroblasts but not by keratinocytes, indicating that these antigens could be used as potential markers for distinguishing between mesenchymal cells and epithelial cell populations.
Annexin A2 was also found to be expressed at low copy number on the cell surface of human PDLSC and gingival fibroblasts, while human keratinocytes lacked any cell surface expression of Annexin A2.
In contrast, sphingosine kinase 1 expression was detected in all the cell types examined using immunocytochemical analysis.
These proteomic studies form the foundation to further define the cell surface protein expression profile of PDLSC in order to better characterise this cell population and help develop novel strategies for the purification of this stem cell population.
American Psychological Association (APA)
Xiong, Jimin& Menicanin, Danijela& Zilm, Peter S.& Marino, Victor& Bartold, P. Mark& Gronthos, Stan. 2016. Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116159
Modern Language Association (MLA)
Xiong, Jimin…[et al.]. Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116159
American Medical Association (AMA)
Xiong, Jimin& Menicanin, Danijela& Zilm, Peter S.& Marino, Victor& Bartold, P. Mark& Gronthos, Stan. Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116159
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116159