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Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy
Joint Authors
Savickiene, Jurate
Treigyte, Grazina
Baronaite, Sandra
Navakauskiene, Ruta
Zentelytė, Aistė
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-10
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Human amniotic-fluid-derived mesenchymal stem cells (AF-MSCs) are interesting for their multilineage differentiation potential and wide range of therapeutic applications due to the ease of culture expansion.
However, MSCs undergo replicative senescence.
So far, the molecular mechanisms that underlie fetal diseases and cell senescence are still poorly understood.
Here, we analyzed senescence-associated morphologic, molecular, and epigenetic characteristics during propagation of MSCs derived from AF of normal and fetus-affected pregnancy.
AF-MSCs cultures from both cell sources displayed quite similar morphology and expression of specific cell surface (CD44, CD90, and CD105) and stemness (Oct4, Nanog, Sox2, and Rex1) markers but had interindividual variability in proliferation capability and time to reach senescence.
Within passages 4 and 8, senescent cultures exhibited typical morphological features, senescence-associated β-galactosidase activity, increased levels of p16, and decreased levels of miR-17 and miR-21 but showed differential expression of p21, p53, and ATM dependently on the onset of cell senescence.
These differences correlated with changes in the level of chromatin modifiers (DNMT1 and HDAC1) and polycomb group proteins (EZH2, SUZ12, and BMI1) paralleling with changes in the expression of repressive histone marks (H3K9me3 and H3K27me3) and stemness markers (Oct4, Nanog, Sox2, and Rex1).
Therefore epigenetic factors are important for AF-MSCs senescence process that may be related with individuality of donor or a fetus malignancy status.
American Psychological Association (APA)
Savickiene, Jurate& Baronaite, Sandra& Zentelytė, Aistė& Treigyte, Grazina& Navakauskiene, Ruta. 2016. Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116171
Modern Language Association (MLA)
Savickiene, Jurate…[et al.]. Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116171
American Medical Association (AMA)
Savickiene, Jurate& Baronaite, Sandra& Zentelytė, Aistė& Treigyte, Grazina& Navakauskiene, Ruta. Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116171