Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy
Joint Authors
Sarnowska, Anna
Lech, Wioletta
Figiel-Dabrowska, Anna
Drela, Katarzyna
Obtulowicz, Patrycja
Noszczyk, Bartlomiej Henryk
Buzanska, Leonora
Domanska-Janik, Krystyna
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-29
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Mesenchymal stem cells (MSC) exhibit enormous heterogeneity which can modify their regenerative properties and therefore influence therapeutic effectiveness as well as safety of these cells transplantation.
In addition the high phenotypic plasticity of MSC population makes it enormously sensitive to any changes in environmental properties including fluctuation in oxygen concentration.
We have shown here that lowering oxygen level far below air atmosphere has a beneficial impact on various parameters characteristic for umbilical cord Wharton Jelly- (WJ-) MSC and adipose tissue- (AD-) derived MSC cultures.
This includes their cellular composition, rate of proliferation, and maintenance of stemness properties together with commitment to cell differentiation toward mesodermal and neural lineages.
In addition, the culture genomic stability increased significantly during long-term cell passaging and eventually protected cells against spontaneous transformation.
Also by comparing of two routinely used methods of MSCs isolation (mechanical versus enzymatic) we have found substantial divergence arising between cell culture properties increasing along the time of cultivation in vitro.
Thus, in this paper we highlight the urgent necessity to develop the more sensitive and selective methods for prediction and control cells fate and functioning during the time of growth in vitro.
American Psychological Association (APA)
Lech, Wioletta& Figiel-Dabrowska, Anna& Sarnowska, Anna& Drela, Katarzyna& Obtulowicz, Patrycja& Noszczyk, Bartlomiej Henryk…[et al.]. 2016. Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116237
Modern Language Association (MLA)
Lech, Wioletta…[et al.]. Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116237
American Medical Association (AMA)
Lech, Wioletta& Figiel-Dabrowska, Anna& Sarnowska, Anna& Drela, Katarzyna& Obtulowicz, Patrycja& Noszczyk, Bartlomiej Henryk…[et al.]. Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116237