Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development
Joint Authors
Failli, Paola
Corti, Francesca
Spinelli, Valentina
Vona, Alessia
Diolaiuti, Lorenzo
Zanardelli, Matteo
Sartiani, Laura
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-20
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Introduction and Aim.
Nitric oxide (NO) can trigger cardiac differentiation of embryonic stem cells (ESCs), indicating a cardiogenic function of the NO synthetizing enzyme(s) (NOS).
However, the involvement of the NO/NOS downstream effectors soluble guanylyl cyclase (sGC) and cGMP activated protein kinase I (PKG-I) is less defined.
Therefore, we assess the involvement of the entire NO/NOS/sGC/PKG-I pathway during cardiac differentiation process.
Methods.
Mouse ESCs were differentiated toward cardiac lineages by hanging drop methodology for 21 days.
NOS/sGC/PKG-I pathway was studied quantifying genes, proteins, enzymatic activities, and effects of inhibition during differentiation.
Percentages of beating embryoid bodies (mEBs) were evaluated as an index of cardiogenesis.
Results and Discussion.
Genes and protein expression of enzymes were increased during differentiation with distinctive kinetics and proteins possessed their enzymatic functions.
Exogenous administered NO accelerated whereas the blockade of PKG-I strongly slowed cardiogenesis.
sGC inhibition was effective only at early stages and NOS blockade ineffective.
Of NOS/sGC/PKG-I pathway, PKG-I seems to play the prominent role in cardiac maturation.
Conclusion.
We concluded that exogenous administered NO and other pharmacological strategies able to increase the activity of PKG-I provide new tools to investigate and promote differentiation of cardiogenic precursors.
American Psychological Association (APA)
Spinelli, Valentina& Vona, Alessia& Corti, Francesca& Diolaiuti, Lorenzo& Zanardelli, Matteo& Sartiani, Laura…[et al.]. 2016. Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116314
Modern Language Association (MLA)
Spinelli, Valentina…[et al.]. Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116314
American Medical Association (AMA)
Spinelli, Valentina& Vona, Alessia& Corti, Francesca& Diolaiuti, Lorenzo& Zanardelli, Matteo& Sartiani, Laura…[et al.]. Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116314