Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming
Joint Authors
Dong, Fulu
Song, Zhenwei
Yu, Jiali
Zhang, Baole
Jiang, BaoChun
Shen, Ying
Lu, Youde
Song, Chunlei
Cong, Peiqing
Liu, Honglin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-13
Country of Publication
Egypt
No. of Pages
10
Abstract EN
The development of induced pluripotent stem cells (iPSCs) has enabled study of the mechanisms underlying cellular reprogramming.
Here, we have studied the dynamic distribution of H2A.Z during induced reprogramming with chromatin immunoprecipitation deep sequencing (ChIP-Seq).
We found that H2A.Z tended to accumulate around transcription start site (TSS) and incorporate in genes with a high transcriptional activity.
GO analysis with H2A.Z incorporated genes indicated that most genes are involved in chromatin assembly or disassembly and chromatin modification both in MEF and Day 7 samples, not in iPSCs.
Furthermore, we detected the highest level of incorporation of H2A.Z around TSS in Day 7 samples compared to MEFs and iPSCs.
GO analysis with only incorporated genes in Day 7 also displayed the function of chromatin remodeling.
So, we speculate H2A.Z may be responsible for chromatin remodeling to enhance the access of transcription factors to genes important for pluripotency.
This study therefore provides a deeper understanding of the mechanisms underlying induced reprogramming.
American Psychological Association (APA)
Dong, Fulu& Song, Zhenwei& Yu, Jiali& Zhang, Baole& Jiang, BaoChun& Shen, Ying…[et al.]. 2015. Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116355
Modern Language Association (MLA)
Dong, Fulu…[et al.]. Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116355
American Medical Association (AMA)
Dong, Fulu& Song, Zhenwei& Yu, Jiali& Zhang, Baole& Jiang, BaoChun& Shen, Ying…[et al.]. Dynamic Changes in Occupancy of Histone Variant H2A.Z during Induced Somatic Cell Reprogramming. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116355