Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp
Joint Authors
Jung, Han-Sung
Kang, Chung-Min
Kim, Hyunok
Song, Je Seon
Choi, Byung-Jai
Kim, Seong-Oh
Moon, Seok-Jun
Choi, Hyung-Jun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-21
Country of Publication
Egypt
No. of Pages
12
Abstract EN
This study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs).
DP tissues were collected from 25 permanent premolars.
UC tissue samples were obtained from three newborns.
Comparative gene profiles were obtained using cDNA microarray analysis and the expression of tooth development-associated and MSC-related genes was assessed by the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).
Genes related to cell proliferation, angiogenesis, and immune responses were expressed at higher levels in UC, whereas genes related to growth factor and receptor activity and signal transduction were more highly expressed in DP.
Although UC and DP tissues exhibited similar expression of surface markers for MSCs, UC showed higher expression of CD29, CD34, CD44, CD73, CD105, CD146, and CD166.
qRT-PCR analysis showed that CD146, CD166, and MYC were expressed 18.3, 8.24, and 1.63 times more highly in UC, whereas the expression of CD34 was 2.15 times higher in DP.
Immunohistochemical staining revealed significant differences in the expression of genes (DSPP, DMP1, and CALB1) related to odontogenesis and angiogenesis in DP.
DP and UC tissue showed similar gene expression, with the usual MSC markers, while they clearly diverged in their differentiation capacity.
American Psychological Association (APA)
Kang, Chung-Min& Kim, Hyunok& Song, Je Seon& Choi, Byung-Jai& Kim, Seong-Oh& Jung, Han-Sung…[et al.]. 2016. Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116382
Modern Language Association (MLA)
Kang, Chung-Min…[et al.]. Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116382
American Medical Association (AMA)
Kang, Chung-Min& Kim, Hyunok& Song, Je Seon& Choi, Byung-Jai& Kim, Seong-Oh& Jung, Han-Sung…[et al.]. Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116382