Improved Protective Effect of Umbilical Cord Stem Cell Transplantation on Cisplatin-Induced Kidney Injury in Mice Pretreated with Antithymocyte Globulin
Joint Authors
Perše, Martina
Mrak-Poljšak, Katjuša
Motaln, Helena
Lah-Turnšek, Tamara
Večerić-Haler, Željka
Erman, Andreja
Kološa, Katja
Škrajnar, Špela
Kranjc, Simona
Arnol, Miha
Cerar, Anton
Lakota, Katja
Sodin-Semrl, Snezna
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Mesenchymal stem cells (MSCs) are recognised as a promising tool to improve renal recovery in experimental models of cisplatin-induced acute kidney injury.
However, these preclinical studies were performed on severely immunodeficient animals.
Here, we investigated whether human umbilical cord derived MSC treatment could equally ameliorate acute kidney injury induced by cisplatin and prolong survival in mice with a normal immune system and those with a suppressed immune system by polyclonal antithymocyte globulin (ATG).
We demonstrated that ATG pretreatment, when followed by MSC transplantation, significantly improved injured renal function parameters, as evidenced by decreased blood urea nitrogen and serum creatinine concentration, as well as improved renal morphology.
This tissue restoration was also supported by increased survival of mice.
The beneficial effects of ATG were associated with reduced level of inflammatory protein serum amyloid A3 and induced antioxidative expression of superoxide dismutase-1 (SOD-1), glutathione peroxidase (GPx), and hem oxygenase-1 (HO-1).
Infused MSCs became localised predominantly in peritubular areas and acted to reduce renal cell death.
In conclusion, these results show that ATG diminished in situ inflammation and oxidative stress associated with cisplatin-induced acute kidney injury, the effects that may provide more favourable microenvironment for MSC action, with consequential synergistic improvements in renal injury and animal survival as compared to MSC treatment alone.
American Psychological Association (APA)
Večerić-Haler, Željka& Erman, Andreja& Cerar, Anton& Motaln, Helena& Kološa, Katja& Lah-Turnšek, Tamara…[et al.]. 2016. Improved Protective Effect of Umbilical Cord Stem Cell Transplantation on Cisplatin-Induced Kidney Injury in Mice Pretreated with Antithymocyte Globulin. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116396
Modern Language Association (MLA)
Večerić-Haler, Željka…[et al.]. Improved Protective Effect of Umbilical Cord Stem Cell Transplantation on Cisplatin-Induced Kidney Injury in Mice Pretreated with Antithymocyte Globulin. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116396
American Medical Association (AMA)
Večerić-Haler, Željka& Erman, Andreja& Cerar, Anton& Motaln, Helena& Kološa, Katja& Lah-Turnšek, Tamara…[et al.]. Improved Protective Effect of Umbilical Cord Stem Cell Transplantation on Cisplatin-Induced Kidney Injury in Mice Pretreated with Antithymocyte Globulin. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116396
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116396