PS1γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells
Joint Authors
Bonfim, Danielle Cabral
Dias, Rhayra B.
Fortuna-Costa, Anneliese
Chicaybam, Leonardo
Lopes, Daiana V.
Dutra, Hélio S.
Borojevic, Radovan
Bonamino, Martin
Mermelstein, Claudia
Rossi, Maria Isabel D.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-08
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Bone marrow stromal cells (BMSCs) are considered a promising tool for bone bioengineering.
However, the mechanisms controlling osteoblastic commitment are still unclear.
Osteogenic differentiation of BMSCs requires the activation of β-catenin signaling, classically known to be regulated by the canonical Wnt pathway.
However, BMSCs treatment with canonical Wnts in vitro does not always result in osteogenic differentiation and evidence indicates that a more complex signaling pathway, involving cadherins, would be required to induce β-catenin signaling in these cells.
Here we showed that Wnt3a alone did not induce TCF activation in BMSCs, maintaining the cells at a proliferative state.
On the other hand, we verified that, upon BMSCs osteoinduction with dexamethasone, cadherins were cleaved by the PS1/γ-secretase complex at the plasma membrane, and this event was associated with an enhanced β-catenin translocation to the nucleus and signaling.
When PS1/γ-secretase activity was inhibited, the osteogenic process was impaired.
Altogether, we provide evidence that PS1/γ-secretase-mediated cadherin cleavage has as an important role in controlling β-catenin signaling during the onset of BMSCs osteogenic differentiation, as part of a complex signaling pathway responsible for cell fate decision.
A comprehensive map of these pathways might contribute to the development of strategies to improve bone repair.
American Psychological Association (APA)
Bonfim, Danielle Cabral& Dias, Rhayra B.& Fortuna-Costa, Anneliese& Chicaybam, Leonardo& Lopes, Daiana V.& Dutra, Hélio S.…[et al.]. 2016. PS1γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1116450
Modern Language Association (MLA)
Bonfim, Danielle Cabral…[et al.]. PS1γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1116450
American Medical Association (AMA)
Bonfim, Danielle Cabral& Dias, Rhayra B.& Fortuna-Costa, Anneliese& Chicaybam, Leonardo& Lopes, Daiana V.& Dutra, Hélio S.…[et al.]. PS1γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1116450
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116450