Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal
Joint Authors
Liu, Shan-Shan
Bai, Yin-Shan
Feng, Li
Dong, Wen-Wei
Li, Yang
Xu, Li-Ping
Ma, Ning-Fang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-27
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Chromodomain helicase/ATPase DNA binding protein 1-like gene (Chd1l) participates in chromatin-dependent processes, including transcriptional activation and DNA repair.
In this study, we have found for the first time that Chd1l is mainly expressed in the testicular tissues of prepubertal and adult mice and colocalized with PLZF, OCT4, and GFRα1 in the neonatal mouse testis and THY1+ undifferentiated spermatogonia or spermatogonial stem cells (SSCs).
Knockdown of endogenous Chd1l in cultured mouse undifferentiated SSCs inhibited the expression levels of Oct4, Plzf, Gfrα1, and Pcna genes, suppressed SSC colony formation, and reduced BrdU incorporation, while increasing SSC apoptosis.
Moreover, the Chd1l gene expression is activated by GDNF in the cultured mouse SSCs, and the GDNF signaling pathway was modulated by endogenous levels of Chd1l; as demonstrated by the gene expression levels of GDNF, inducible transcripts Etv5, Bcl6b, Pou3f, and Lhx1, but not that of GDNF-independent gene, Taf4b, were significantly downregulated by Chd1l knockdown in mouse SSCs.
Taken together, this study provides the first evidence to support the notion that Chd1l is an intrinsic and novel regulator for SSC survival and self-renewal, and it exerts such regulation at least partially through a GDNF signaling pathway.
American Psychological Association (APA)
Liu, Shan-Shan& Bai, Yin-Shan& Feng, Li& Dong, Wen-Wei& Li, Yang& Xu, Li-Ping…[et al.]. 2016. Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116488
Modern Language Association (MLA)
Liu, Shan-Shan…[et al.]. Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116488
American Medical Association (AMA)
Liu, Shan-Shan& Bai, Yin-Shan& Feng, Li& Dong, Wen-Wei& Li, Yang& Xu, Li-Ping…[et al.]. Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116488