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Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors
Joint Authors
Verfaillie, Catherine M.
Himmelreich, Uwe
Leten, Cindy
Trekker, Jesse
Roobrouck, Valerie D.
Dresselaers, Tom
Vande Velde, Greetje
Struys, Tom
Lambrichts, Ivo
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Tumor infiltrating stem cells have been suggested as a vehicle for the delivery of a suicide gene towards otherwise difficult to treat tumors like glioma.
We have used herpes simplex virus thymidine kinase expressing human multipotent adult progenitor cells in two brain tumor models (hU87 and Hs683) in immune-compromised mice.
In order to determine the best time point for the administration of the codrug ganciclovir, the stem cell distribution and viability were monitored in vivo using bioluminescence (BLI) and magnetic resonance imaging (MRI).
Treatment was assessed by in vivo BLI and MRI of the tumors.
We were able to show that suicide gene therapy using HSV-tk expressing stem cells can be followed in vivo by MRI and BLI.
This has the advantage that (1) outliers can be detected earlier, (2) GCV treatment can be initiated based on stem cell distribution rather than on empirical time points, and (3) a more thorough follow-up can be provided prior to and after treatment of these animals.
In contrast to rodent stem cell and tumor models, treatment success was limited in our model using human cell lines.
This was most likely due to the lack of immune components in the immune-compromised rodents.
American Psychological Association (APA)
Leten, Cindy& Trekker, Jesse& Struys, Tom& Roobrouck, Valerie D.& Dresselaers, Tom& Vande Velde, Greetje…[et al.]. 2016. Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1116500
Modern Language Association (MLA)
Leten, Cindy…[et al.]. Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1116500
American Medical Association (AMA)
Leten, Cindy& Trekker, Jesse& Struys, Tom& Roobrouck, Valerie D.& Dresselaers, Tom& Vande Velde, Greetje…[et al.]. Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1116500
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116500