Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture
Joint Authors
Schmal, Olga
Seifert, Jan
Schäffer, Tilman E.
Walter, Christina B.
Aicher, Wilhelm K.
Klein, Gerd
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Efficient ex vivo expansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition.
Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported.
Here, we describe a simplified 3D hanging drop model for the coculture of cord blood-derived CD34+ hematopoietic stem and progenitor cells (HSPCs) with bone marrow-derived mesenchymal stromal cells (MSCs).
When seeded as a mixed cell suspension, MSCs segregated into tight spheroids.
Despite the high expression of niche-specific extracellular matrix components by spheroid-forming MSCs, HSPCs did not migrate into the spheroids in the initial phase of coculture, indicating strong homotypic interactions of MSCs.
After one week, however, HSPC attachment increased considerably, leading to spheroid collapse as demonstrated by electron microscopy and immunofluorescence staining.
In terms of HSPC proliferation, the conventional 2D coculture system was superior to the hanging drop model.
Furthermore, expansion of primitive hematopoietic progenitors was more favored in 2D than in 3D, as analyzed in colony-forming assays.
Conclusively, our data demonstrate that MSCs, when arranged with a spread (monolayer) shape, exhibit better HSPC supportive qualities than spheroid-forming MSCs.
Therefore, 3D systems are not necessarily superior to traditional 2D culture in this regard.
American Psychological Association (APA)
Schmal, Olga& Seifert, Jan& Schäffer, Tilman E.& Walter, Christina B.& Aicher, Wilhelm K.& Klein, Gerd. 2015. Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116505
Modern Language Association (MLA)
Schmal, Olga…[et al.]. Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116505
American Medical Association (AMA)
Schmal, Olga& Seifert, Jan& Schäffer, Tilman E.& Walter, Christina B.& Aicher, Wilhelm K.& Klein, Gerd. Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116505
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116505