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Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment
Joint Authors
Paik, Man-Jeong
Shin, Tae Hwan
Phukan, Geetika
Shim, Jeom Soon
Nguyen, Duc-Toan
Kim, Yongman
Lee, Hyeon-Seong
Oh-Lee, Justin D.
Lee, Gwang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-16
Country of Publication
Egypt
No. of Pages
11
Abstract EN
We investigated changes in PA levels by the treatment of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in ischemic stroke in rat brain model and in cultured neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation (OGD).
In ischemic rat model, transient middle cerebral artery occlusion (MCAo) was performed for 2 h, followed by intravenous transplantation of hBM-MSCs or phosphate-buffered saline (PBS) the day following MCAo.
Metabolic profiling analysis of PAs was examined in brains from three groups: control rats, PBS-treated MCAo rats (MCAo), and hBM-MSCs-treated MCAo rats (MCAo + hBM-MSCs).
In ischemic cell model, SH-SY5Y cells were exposed to OGD for 24 h, treated with hBM-MSCs (OGD + hBM-MSCs) prior to continued aerobic incubation, and then samples were collected after coculture for 72 h.
In the in vivo MCAo ischemic model, levels of some PAs in brain samples of the MCAo and MCAo + hBM-MSCs groups were significantly different from those of the control group.
In particular, putrescine, cadaverine, and spermidine in brain tissues of the MCAo + hBM-MSCs group were significantly reduced in comparison to those in the MCAo group.
In the in vitro OGD system, N 1 -acetylspermidine, spermidine, N 1 -acetylspermine, and spermine in cells of the OGD + hBM-MSCs group were significantly reduced compared to those of OGD group.
American Psychological Association (APA)
Shin, Tae Hwan& Phukan, Geetika& Shim, Jeom Soon& Nguyen, Duc-Toan& Kim, Yongman& Oh-Lee, Justin D.…[et al.]. 2016. Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116587
Modern Language Association (MLA)
Shin, Tae Hwan…[et al.]. Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116587
American Medical Association (AMA)
Shin, Tae Hwan& Phukan, Geetika& Shim, Jeom Soon& Nguyen, Duc-Toan& Kim, Yongman& Oh-Lee, Justin D.…[et al.]. Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116587