Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4
Joint Authors
Li, Yang
Zhou, Shixin
Liu, Yinan
Feng, Ruopeng
Wang, Caiyun
Jiang, Sibo
Zhang, Xiaoyan
Lan, Feng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-16
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Induced pluripotent stem (iPS) cells have been generated from human somatic cells by ectopic expression of four Yamanaka factors.
Here, we report that Survivin, an apoptosis inhibitor, can enhance iPS cells generation from human neural progenitor cells (NPCs) together with one factor OCT4 (1F-OCT4-Survivin).
Compared with 1F-OCT4, Survivin accelerates the process of reprogramming from human NPCs.
The neurocyte-originated induced pluripotent stem (NiPS) cells generated from 1F-OCT4-Survivin resemble human embryonic stem (hES) cells in morphology, surface markers, global gene expression profiling, and epigenetic status.
Survivin keeps high expression in both iPS and ES cells.
During the process of NiPS cell to neural cell differentiation, the expression of Survivin is rapidly decreased in protein level.
The mechanism of Survivin promotion of reprogramming efficiency from NPCs may be associated with stabilization of β-catenin in WNT signaling pathway.
This hypothesis is supported by experiments of RT-PCR, chromatin immune-precipitation, and Western blot in human ES cells.
Our results showed overexpression of Survivin could improve the efficiency of reprogramming from NPCs to iPS cells by one factor OCT4 through stabilization of the key molecule, β-catenin.
American Psychological Association (APA)
Zhou, Shixin& Liu, Yinan& Feng, Ruopeng& Wang, Caiyun& Jiang, Sibo& Zhang, Xiaoyan…[et al.]. 2016. Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116622
Modern Language Association (MLA)
Zhou, Shixin…[et al.]. Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116622
American Medical Association (AMA)
Zhou, Shixin& Liu, Yinan& Feng, Ruopeng& Wang, Caiyun& Jiang, Sibo& Zhang, Xiaoyan…[et al.]. Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116622
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116622