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Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells
Joint Authors
Zhang, Peihua
Li, Jin
Qi, Yawei
Tang, Xudong
Duan, Jianfeng
Liu, Li
Wu, Zeyong
Liang, Jie
Li, Jiangfeng
Wang, Xian
Zeng, Guofang
Liu, Hongwei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-27
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a multifunctional matrix metalloproteinase, and it is involved in the regulation of cell proliferation and apoptosis in various cell types.
However, little is known about the effect of TIMP-1 expression on the proliferation of adipose-derived stem cells (ADSCs).
Therefore, TIMP-1 expression in the ADSCs was firstly detected by western blotting, and TIMP-1 gene was knocked down by lentivirus-mediated shRNA.
Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively.
Cell cycle progression was determined by flow cytometry.
The changes of p51, p21, cyclin E, cyclin-dependent kinase 2 (CDK2), and P-CDK2 caused by TIMP-1 knockdown were detected by western blotting.
The results indicated that ADSCs highly expressed TIMP-1 protein, and the knockdown of TIMP-1 inhibited cell proliferation and arrested cell cycle progression at G1 phase in the ADSCs possibly through the upregulation of p53, p21, and P-CDK2 protein levels and concurrent downregulation of cyclin E and CDK2 protein levels.
These findings suggest that TIMP-1 works as a positive regulator of cell proliferation in ADSCs.
American Psychological Association (APA)
Zhang, Peihua& Li, Jin& Qi, Yawei& Tang, Xudong& Duan, Jianfeng& Liu, Li…[et al.]. 2016. Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116633
Modern Language Association (MLA)
Zhang, Peihua…[et al.]. Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1116633
American Medical Association (AMA)
Zhang, Peihua& Li, Jin& Qi, Yawei& Tang, Xudong& Duan, Jianfeng& Liu, Li…[et al.]. Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116633
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116633