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Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use
Joint Authors
Petry, Florian
Smith, J. Robert
Leber, Jasmin
Salzig, Denise
Weiss, Mark L.
Czermak, Peter
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-08
Country of Publication
Egypt
No. of Pages
12
Abstract EN
The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine.
Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues.
For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system.
For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells.
The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask.
The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.
American Psychological Association (APA)
Petry, Florian& Smith, J. Robert& Leber, Jasmin& Salzig, Denise& Czermak, Peter& Weiss, Mark L.. 2016. Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116657
Modern Language Association (MLA)
Petry, Florian…[et al.]. Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116657
American Medical Association (AMA)
Petry, Florian& Smith, J. Robert& Leber, Jasmin& Salzig, Denise& Czermak, Peter& Weiss, Mark L.. Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116657