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Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells
Joint Authors
Tettamanti, G.
Sampaolesi, Maurilio
Anastasia, Luigi
Palazzolo, Giacomo
Quattrocelli, Mattia
Toelen, Jaan
Dominici, Roberto
Barthelemy, Inès
Blot, Stephane
Gijsbers, Rik
Cassano, Marco
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-23
Country of Publication
Egypt
No. of Pages
13
Abstract EN
The Duchenne and Becker muscular dystrophies are caused by mutation of dystrophin gene and primarily affect skeletal and cardiac muscles.
Cardiac involvement in dystrophic GRMD dogs has been demonstrated by electrocardiographic studies with the onset of a progressive cardiomyopathy similar to the cardiac disease in DMD patients.
In this respect, GRMD is a useful model to explore cardiac and skeletal muscle pathogenesis and for developing new therapeutic protocols.
Here we describe a protocol to convert GRMD canine fibroblasts isolated from heart and skin into induced cardiac-like myocytes (ciCLMs).
We used a mix of transcription factors (GATA4, HAND2, TBX5, and MEF2C), known to be able to differentiate mouse and human somatic cells into ciCLMs.
Exogenous gene expression was obtained using four lentiviral vectors carrying transcription factor genes and different resistance genes.
Our data demonstrate a direct switch from fibroblast into ciCLMs with no activation of early cardiac genes.
ciCLMs were unable to contract spontaneously, suggesting, differently from mouse and human cells, an incomplete differentiation process.
However, when transplanted in neonatal hearts of SCID/Beige mice, ciCLMs participate in cardiac myogenesis.
American Psychological Association (APA)
Palazzolo, Giacomo& Quattrocelli, Mattia& Toelen, Jaan& Dominici, Roberto& Anastasia, Luigi& Tettamanti, G.…[et al.]. 2015. Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116679
Modern Language Association (MLA)
Palazzolo, Giacomo…[et al.]. Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116679
American Medical Association (AMA)
Palazzolo, Giacomo& Quattrocelli, Mattia& Toelen, Jaan& Dominici, Roberto& Anastasia, Luigi& Tettamanti, G.…[et al.]. Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116679
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116679