Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury
Joint Authors
Deng, Chunhua
Chen, Xin
Yang, Qiyun
Zheng, Tao
Bian, Jun
Sun, Xiangzhou
Shi, Yanan
Liang, Xiaoyan
Gao, Guoquan
Liu, Guihua
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-13
Country of Publication
Egypt
No. of Pages
12
Abstract EN
The paracrine effect is the major mechanism of stem cell therapy.
However, the details of the effect’s mechanism remain unknown.
The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to determine its mechanism.
Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU-) labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP).
The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF) in vitro.
The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI) group for 14 days and then gradually decreased.
On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS-) treated group.
Moreover, the neuronal nitric oxide synthase (nNOS) and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased.
Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group.
This study demonstrated that intracavernous injection of ADSCs improved erectile function, repaired the nerve, and corrected penile fibrosis.
One potential mechanism is the PEDF secretion of ADSCs and subsequent PI3K/Akt pathway activation.
American Psychological Association (APA)
Chen, Xin& Yang, Qiyun& Zheng, Tao& Bian, Jun& Sun, Xiangzhou& Shi, Yanan…[et al.]. 2015. Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116712
Modern Language Association (MLA)
Chen, Xin…[et al.]. Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116712
American Medical Association (AMA)
Chen, Xin& Yang, Qiyun& Zheng, Tao& Bian, Jun& Sun, Xiangzhou& Shi, Yanan…[et al.]. Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116712