Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells
Joint Authors
Inthanon, Kewalin
Techaikool, Pimwalan
Khaniyao, Vorathep
Bernstein, Audrey M.
Wongkham, Weerah
Punyodom, Winita
Daranarong, Donraporn
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
16
Abstract EN
Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale.
An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections.
We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning.
Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile.
The hydrophilic properties of the membranes were 35–40% higher than those of a standard PLCL and commercial polystyrene (PS).
The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N–H, and C–N, onto their surfaces.
Degradation of the membranes was controllable, depending on the content proportion of SC.
Results of thermogram indicated the superior stability and crystallinity of the membranes.
These membranes enhanced human Wharton’s jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK).
On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin.
These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.
American Psychological Association (APA)
Inthanon, Kewalin& Daranarong, Donraporn& Techaikool, Pimwalan& Punyodom, Winita& Khaniyao, Vorathep& Bernstein, Audrey M.…[et al.]. 2015. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116736
Modern Language Association (MLA)
Inthanon, Kewalin…[et al.]. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1116736
American Medical Association (AMA)
Inthanon, Kewalin& Daranarong, Donraporn& Techaikool, Pimwalan& Punyodom, Winita& Khaniyao, Vorathep& Bernstein, Audrey M.…[et al.]. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116736