Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model
Joint Authors
Yang, Bing
Parsha, Kaushik
Schaar, Krystal
Satani, Nikunj
Xi, Xiaopei
Aronowski, Jaroslaw
Savitz, Sean I.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
7
Abstract EN
The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke.
Autologous applications raise the possibility that MNCs could potentially be stored as a banked source.
There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models.
In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs.
BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group.
BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test.
We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs.
Cryopreservation also alters the cellular composition of various subpopulations within the MNCs.
However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.
American Psychological Association (APA)
Yang, Bing& Parsha, Kaushik& Schaar, Krystal& Satani, Nikunj& Xi, Xiaopei& Aronowski, Jaroslaw…[et al.]. 2016. Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model. Stem Cells International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1116844
Modern Language Association (MLA)
Yang, Bing…[et al.]. Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1116844
American Medical Association (AMA)
Yang, Bing& Parsha, Kaushik& Schaar, Krystal& Satani, Nikunj& Xi, Xiaopei& Aronowski, Jaroslaw…[et al.]. Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1116844
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116844