Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Joint Authors
Tan, Chengbo
Shichinohe, Hideo
Wang, Zifeng
Hamauchi, Shuji
Abumiya, Takeo
Nakayama, Naoki
Kazumata, Ken
Ito, Tsuneo
Kudo, Kohsuke
Takamoto, Shigeru
Houkin, Kiyohiro
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-20
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke.
The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs).
Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL).
The PL mixtures (mPL) were made from three different sPL.
Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA).
The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes.
The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain.
MRI and histological analysis were performed.
Consequently, nine lots of sPL and three mPL were prepared.
ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens.
Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS).
No contradiction in cell surface markers and no chromosomal aberrations were found.
The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain.
In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke.
American Psychological Association (APA)
Tan, Chengbo& Shichinohe, Hideo& Wang, Zifeng& Hamauchi, Shuji& Abumiya, Takeo& Nakayama, Naoki…[et al.]. 2016. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116869
Modern Language Association (MLA)
Tan, Chengbo…[et al.]. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116869
American Medical Association (AMA)
Tan, Chengbo& Shichinohe, Hideo& Wang, Zifeng& Hamauchi, Shuji& Abumiya, Takeo& Nakayama, Naoki…[et al.]. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116869