Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage
Joint Authors
Schendzielorz, P.
Froelich, K.
Rak, K.
Gehrke, T.
Scherzad, A.
Hagen, R.
Radeloff, A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-08
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Adipose-derived stem cells (ASCs) have been extensively studied in the field of stem cell research and possess numerous clinical applications.
Cell labeling is an essential component of various experimental protocols and Hoechst 33342 (H33342) represents a cost-effective and easy methodology for live staining.
The purpose of this study was to evaluate the labeling of rat ASCs with two different concentrations of H33342 (0.5 μg/mL and 5 μg/mL), with particular regard to usability, interference with cell properties, and potential DNA damage.
Hoechst 33342 used at a low concentration of 0.5 μg/mL did not significantly affect cell proliferation, viability, or differentiation potential of the ASCs, nor did it cause any significant DNA damage as measured by the olive tail moment.
High concentrations of 5 μg/mL H33342, however, impaired the proliferation and viability of the ASCs, and considerable DNA damage was observed.
Undesirable colabeling of unlabeled cocultivated cells was seen in particular with higher concentrations of H33342, independent of varying washing procedures.
Hence, H33342 labeling with lower concentrations represents a usable method, which does not affect the tested cell properties.
However, the colabeling of adjacent cells is a drawback of the technique.
American Psychological Association (APA)
Schendzielorz, P.& Froelich, K.& Rak, K.& Gehrke, T.& Scherzad, A.& Hagen, R.…[et al.]. 2016. Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116942
Modern Language Association (MLA)
Schendzielorz, P.…[et al.]. Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116942
American Medical Association (AMA)
Schendzielorz, P.& Froelich, K.& Rak, K.& Gehrke, T.& Scherzad, A.& Hagen, R.…[et al.]. Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116942
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116942