FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells
Joint Authors
Rojas-López, Marlon
Pérez-Ishiwara, David Guillermo
Vazquez-Zapien, Gustavo Jesus
Mata-Miranda, Monica Maribel
Delgado-Macuil, Raul Jacobo
Sánchez Monroy, Virginia
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-29
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Some of the greatest challenges in stem cells (SCs) biology and regenerative medicine are differentiation control of SCs and ensuring the purity of differentiated cells.
In this work, we differentiated mouse pluripotent stem cells (mPSCs) toward pancreatic cells characterizing this differentiation process by molecular and spectroscopic technics.
Both mPSCs and Differentiated Pancreatic Cells (DPCs) were subjected to a genetic, phenotypic, and biochemical analysis by real-time quantitative PCR (RT-qPCR), immunocytochemistry, and Fourier Transform Infrared (FTIR) spectroscopy.
Cultured mPCSs expressed pluripotent genes and proteins (Nanog and SOX2).
DPCs expressed endodermal genes (SOX17 and Pdx1) at day 11, an inductor gene of embryonic pancreas development (Pdx1) at day 17 and pancreas genes and proteins (Insulin and Glucagon) at day 21 of differentiation.
Likewise, FTIR spectra of mPSCs and DPCs at different maturation stages (11, 17, and 21 days) were obtained and showed absorption bands related with different types of biomolecules.
These FTIR spectra exhibited significant spectral changes agreeing with the differentiation process, particularly in proteins and nucleic acids bands.
In conclusion, the obtained DPCs passed through the chronological stages of embryonic pancreas development and FTIR spectra provide a new biophysical parameter based on molecular markers indicating the differentiation process of mPSCs to specialized cells.
American Psychological Association (APA)
Vazquez-Zapien, Gustavo Jesus& Mata-Miranda, Monica Maribel& Sánchez Monroy, Virginia& Delgado-Macuil, Raul Jacobo& Pérez-Ishiwara, David Guillermo& Rojas-López, Marlon. 2016. FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116953
Modern Language Association (MLA)
Vazquez-Zapien, Gustavo Jesus…[et al.]. FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116953
American Medical Association (AMA)
Vazquez-Zapien, Gustavo Jesus& Mata-Miranda, Monica Maribel& Sánchez Monroy, Virginia& Delgado-Macuil, Raul Jacobo& Pérez-Ishiwara, David Guillermo& Rojas-López, Marlon. FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116953