Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering
Joint Authors
Wei, Xiao-Qing
Jiang, Wenkai
Song, Bing
Sloan, Alastair J.
Ni, Longxing
Alraies, Amr
Liu, Qian
Gudla, Vijay
Oni, Julia
Agarwal, Meena
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering.
Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder.
It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents.
However, most of these studies are focused on the vascular SMCs.
The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation.
We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1).
After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time.
In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs.
These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering.
American Psychological Association (APA)
Song, Bing& Jiang, Wenkai& Alraies, Amr& Liu, Qian& Gudla, Vijay& Oni, Julia…[et al.]. 2016. Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117022
Modern Language Association (MLA)
Song, Bing…[et al.]. Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117022
American Medical Association (AMA)
Song, Bing& Jiang, Wenkai& Alraies, Amr& Liu, Qian& Gudla, Vijay& Oni, Julia…[et al.]. Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117022
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117022