![](/images/graphics-bg.png)
Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts
Joint Authors
Verfaillie, Catherine M.
Sambathkumar, Rangarajan
Kalo, Eric
Van Rossom, Rob
Faas, Marijke M.
de Vos, Paul
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Reprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes.
We demonstrated that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM) induced expression of a number of definitive endoderm and early and late pancreatic marker genes.
When CRM was omitted, endoderm/pancreatic marker genes were not induced.
Furthermore, treatment with DNA methyltransferase inhibitor (DNMTi) 5-azacytidine (5AZA) CRM did not affect gene expression changes, and when 5AZA was combined with TSA, no further increase in gene expression of endoderm, pancreatic endoderm, and endocrine markers was seen over levels induced with TSA alone.
Interestingly, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts.
Upon removal of TSA-CRM, the endoderm/pancreatic gene expression profile returned to baseline.
Our findings underscore the role epigenetic modification in transdifferentiation of one somatic cell into another.
However, full reprogramming of fibroblasts to β-cells will require combination of this approach with TF overexpression and/or culture of the partially reprogrammed cells under β -cell specific conditions.
American Psychological Association (APA)
Sambathkumar, Rangarajan& Kalo, Eric& Van Rossom, Rob& Faas, Marijke M.& de Vos, Paul& Verfaillie, Catherine M.. 2016. Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts. Stem Cells International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117149
Modern Language Association (MLA)
Sambathkumar, Rangarajan…[et al.]. Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1117149
American Medical Association (AMA)
Sambathkumar, Rangarajan& Kalo, Eric& Van Rossom, Rob& Faas, Marijke M.& de Vos, Paul& Verfaillie, Catherine M.. Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117149
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117149