Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4
Joint Authors
Jung, Jieun
Choi, Jong Ho
Lee, Yun Bin
Hwang, Seong Gyu
Oh, IL-Hoan
Kim, Gi Jin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-04
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Although hypoxic environments have been known to regulate the migratory ability of bone marrow-derived mesenchymal stem cells (BM-MSCs), which is a critical factor for maximizing the therapeutic effect, the underlying mechanisms remain unclear.
Therefore, we aimed to confirm the effect of hypoxia-inducible factor-1α (HIF-1α) on the migration of BM-MSCs and to analyze the interaction between HIF-1α and integrin-mediated signals.
Hypoxia-activated HIF-1α significantly increased BM-MSC migration.
The expression of integrin α 4 was decreased in BM-MSCs by increased HIF-1α under hypoxia, whereas the expression of Rho-associated kinase 1 (ROCK1) and Rac1/2/3 was increased.
After downregulation of HIF-1α by YC-1, which is an inhibitor of HIF-1α, BM-MSC migration was decreased via upregulation of integrin α 4 and downregulation of ROCK1 and Rac1/2/3.
Knockdown of integrin α 4 by integrin α 4 siRNA (siITGA4) treatment increased BM-MSC migration by upregulation of ROCK1, Rac1/2/3, and matrix metalloproteinase-2 regardless of oxygen tension.
Moreover, siITGA4 treatment increased HIF-1α expression and augmented the translocation of HIF-1α into the nucleus under hypoxia.
Taken together, the alternative expression of HIF-1α induced by microenvironment factors, such as hypoxia and integrin α 4, may regulate the migration of BM-MSCs.
These findings may provide insights to the underlying mechanisms of BM-MSC migration for successful stem cell-based therapy.
American Psychological Association (APA)
Choi, Jong Ho& Lee, Yun Bin& Jung, Jieun& Hwang, Seong Gyu& Oh, IL-Hoan& Kim, Gi Jin. 2016. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117172
Modern Language Association (MLA)
Choi, Jong Ho…[et al.]. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117172
American Medical Association (AMA)
Choi, Jong Ho& Lee, Yun Bin& Jung, Jieun& Hwang, Seong Gyu& Oh, IL-Hoan& Kim, Gi Jin. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117172