Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4

Joint Authors

Jung, Jieun
Choi, Jong Ho
Lee, Yun Bin
Hwang, Seong Gyu
Oh, IL-Hoan
Kim, Gi Jin

Source

Stem Cells International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-01-04

Country of Publication

Egypt

No. of Pages

11

Abstract EN

Although hypoxic environments have been known to regulate the migratory ability of bone marrow-derived mesenchymal stem cells (BM-MSCs), which is a critical factor for maximizing the therapeutic effect, the underlying mechanisms remain unclear.

Therefore, we aimed to confirm the effect of hypoxia-inducible factor-1α (HIF-1α) on the migration of BM-MSCs and to analyze the interaction between HIF-1α and integrin-mediated signals.

Hypoxia-activated HIF-1α significantly increased BM-MSC migration.

The expression of integrin α 4 was decreased in BM-MSCs by increased HIF-1α under hypoxia, whereas the expression of Rho-associated kinase 1 (ROCK1) and Rac1/2/3 was increased.

After downregulation of HIF-1α by YC-1, which is an inhibitor of HIF-1α, BM-MSC migration was decreased via upregulation of integrin α 4 and downregulation of ROCK1 and Rac1/2/3.

Knockdown of integrin α 4 by integrin α 4 siRNA (siITGA4) treatment increased BM-MSC migration by upregulation of ROCK1, Rac1/2/3, and matrix metalloproteinase-2 regardless of oxygen tension.

Moreover, siITGA4 treatment increased HIF-1α expression and augmented the translocation of HIF-1α into the nucleus under hypoxia.

Taken together, the alternative expression of HIF-1α induced by microenvironment factors, such as hypoxia and integrin α 4, may regulate the migration of BM-MSCs.

These findings may provide insights to the underlying mechanisms of BM-MSC migration for successful stem cell-based therapy.

American Psychological Association (APA)

Choi, Jong Ho& Lee, Yun Bin& Jung, Jieun& Hwang, Seong Gyu& Oh, IL-Hoan& Kim, Gi Jin. 2016. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117172

Modern Language Association (MLA)

Choi, Jong Ho…[et al.]. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117172

American Medical Association (AMA)

Choi, Jong Ho& Lee, Yun Bin& Jung, Jieun& Hwang, Seong Gyu& Oh, IL-Hoan& Kim, Gi Jin. Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117172

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1117172