Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells
Joint Authors
Liu, Quanwen
Shen, Yi
Chen, Jiarong
Ding, Jie
Tang, Zihua
Zhang, Cui
Chen, Jianling
Li, Liang
Chen, Ping
Wang, Jinfu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
14
Abstract EN
In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells.
Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum.
Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles.
The hair-cell-like cells exhibited rapid permeation of FM1-43FX.
The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells.
The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment.
American Psychological Association (APA)
Liu, Quanwen& Shen, Yi& Chen, Jiarong& Ding, Jie& Tang, Zihua& Zhang, Cui…[et al.]. 2015. Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117204
Modern Language Association (MLA)
Liu, Quanwen…[et al.]. Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117204
American Medical Association (AMA)
Liu, Quanwen& Shen, Yi& Chen, Jiarong& Ding, Jie& Tang, Zihua& Zhang, Cui…[et al.]. Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117204