Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent
Joint Authors
Skutella, Thomas
Azizi, Hossein
Conrad, Sabine
Hinz, Ursula
Asgari, Behrouz
Nanus, Daniel
Peterziel, Heike
Hajizadeh Moghaddam, Akbar
Baharvand, Hossein
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-09
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells.
While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age.
Similarly, there have been differences in the literature about the duration of cultures during which ESC-like cells may appear.
We demonstrate the possibility to derive ESC-like cells from SSC cultures until they reach adolescence or up to 7 weeks of age, but we point out the impossibility to derive these cells from older, mature adult mice.
The inability of real adult SSCs to shift to a pluripotent state coincides with a decline in expression of the core pluripotency genes Oct4, Nanog, and Sox2 in SSCs with age.
At the same time genes of the spermatogonial differentiation pathway increase.
The generated ESC-like cells were similar to ESCs and express pluripotency markers.
In vitro they differentiate into all three germ lineages; they form complex teratomas after transplantation in SCID mice and produce chimeric mice.
American Psychological Association (APA)
Azizi, Hossein& Conrad, Sabine& Hinz, Ursula& Asgari, Behrouz& Nanus, Daniel& Peterziel, Heike…[et al.]. 2015. Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117211
Modern Language Association (MLA)
Azizi, Hossein…[et al.]. Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1117211
American Medical Association (AMA)
Azizi, Hossein& Conrad, Sabine& Hinz, Ursula& Asgari, Behrouz& Nanus, Daniel& Peterziel, Heike…[et al.]. Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117211
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117211