Regulatory Mechanisms of the IhhPTHrP Signaling Pathway in Fibrochondrocytes in Entheses of Pig Achilles Tendon
Joint Authors
Han, Xuesong
Zhuang, Yanfeng
Zhang, Zhihong
Guo, Lin
Wang, Wanming
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-22
Country of Publication
Egypt
No. of Pages
12
Abstract EN
This study is aimed at exploring the effect of stress stimulation on the proliferation and differentiation of fibrochondrocytes in entheses mediated via the Indian hedgehog (Ihh)/parathyroid hormone-related protein (PTHrP) signaling pathway.
Differential stress stimulation on fibrochondrocytes in entheses was imposed.
Gene expression and protein levels of signaling molecules including collagen type I (Col I), Col II, Col X, Ihh, and PTHrP in the cytoplasm of fibrochondrocytes were detected.
Ihh signal blocking group was set up using Ihh signaling pathway-specific blocking agent cyclopamine.
PTHrP enhancement group was set up using PTHrP reagent.
Ihh/PTHrP double intervention group, as well as control group, was included to study the regulatory mechanisms of the Ihh/PTHrP signaling pathway in fibrochondrocytes.
Under low cyclic stress tensile (CTS), PTHrP, Col I, and Col II gene expression and protein synthesis increased.
Under high CTS, Ihh and Col X gene expression and protein synthesis increased.
Blocking Ihh signaling with cyclopamine resulted in reduced PTHrP gene expression and protein synthesis and increased Col X gene expression and protein synthesis.
Ihh and PTHrP coregulate fibrochondrocyte proliferation and differentiation in entheses through negative feedback regulation.
Fibrochondrocyte is affected by the CTS.
This phenomenon is regulated by stress stimulation through the Ihh/PTHrP signaling pathway.
American Psychological Association (APA)
Han, Xuesong& Zhuang, Yanfeng& Zhang, Zhihong& Guo, Lin& Wang, Wanming. 2016. Regulatory Mechanisms of the IhhPTHrP Signaling Pathway in Fibrochondrocytes in Entheses of Pig Achilles Tendon. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117213
Modern Language Association (MLA)
Han, Xuesong…[et al.]. Regulatory Mechanisms of the IhhPTHrP Signaling Pathway in Fibrochondrocytes in Entheses of Pig Achilles Tendon. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1117213
American Medical Association (AMA)
Han, Xuesong& Zhuang, Yanfeng& Zhang, Zhihong& Guo, Lin& Wang, Wanming. Regulatory Mechanisms of the IhhPTHrP Signaling Pathway in Fibrochondrocytes in Entheses of Pig Achilles Tendon. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117213