Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus
Joint Authors
Sang, Chenglin
Cao, Xuecheng
Chen, Fangjing
Yang, Xuekang
Zhang, Yongxian
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Objective.
Nucleus pulposus (NP) and annulus fibrosus (AF) are two main components of intervertebral disc (IVD).
We aimed to figure out whether NP and AF also contain stem cells and whether these stem cells share common properties with chondrocytes and/or fibroblasts in their phenotypes or whether they are completely different types of cells with different characteristics.
Design.
The disk cells were isolated from AF and NP tissues of the same lumbar spine of the rabbits.
The properties of these disk cells were characterized by their morphology, population doubling time (PDT), stem cell marker expression, and multidifferentiation potential using tissue culture techniques, immunocytochemistry, and RT-PCR.
Results.
Both disk cells formed colonies in culture and expressed stem cell markers, nucleostemin, Oct-4, SSEA-4, and Stro-1, at early passages.
However, after 5 passages, AFSCs became elongated and NPSCs appeared senescent.
Conclusion.
This study indicated that IVD contains stem cells and the characteristics of AFSCs and NPSCs are intrinsically different.
The findings of this study may provide basic scientific data for understanding the properties of IVD cells and the mechanisms of lower back pain.
American Psychological Association (APA)
Sang, Chenglin& Cao, Xuecheng& Chen, Fangjing& Yang, Xuekang& Zhang, Yongxian. 2016. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117224
Modern Language Association (MLA)
Sang, Chenglin…[et al.]. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117224
American Medical Association (AMA)
Sang, Chenglin& Cao, Xuecheng& Chen, Fangjing& Yang, Xuekang& Zhang, Yongxian. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117224