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Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity
Joint Authors
Agathopoulos, S.
Maltabe, Violetta A.
Barka, Eleonora
Kontonika, Marianthi
Florou, Dimitra
Kouvara-Pritsouli, Maria
Roumpi, Maria
Kouklis, Panos
Kolettis, Theofilos M.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-22
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Embryonic Stem (ES) or induced Pluripotent Stem (iPS) cells are important sources for cardiomyocyte generation, targeted for regenerative therapies.
Several in vitro protocols are currently utilized for their differentiation, but the value of cell-based approaches remains unclear.
Here, we characterized a cardiovascular progenitor population derived during ES differentiation, after selection based on VE-cadherin promoter (Pvec) activity.
ESCs were genetically modified with an episomal vector, allowing the expression of puromycin resistance gene, under Pvec activity.
Puromycin-surviving cells displayed cardiac and endothelial progenitor cells characteristics.
Expansion and self-renewal of this cardiac and endothelial dual-progenitor population (CEDP) were achieved by Wnt/β-catenin pathway activation.
CEDPs express early cardiac developmental stage-specific markers but not markers of differentiated cardiomyocytes.
Similarly, CEDPs express endothelial markers.
However, CEDPs can undergo differentiation predominantly to cTnT+ (~47%) and VE-cadherin+ (~28%) cells.
Transplantation of CEDPs in the left heart ventricle of adult rats showed that CEDPs-derived cells survive and differentiate in vivo for at least 14 days after transplantation.
A novel, dual-progenitor population was isolated during ESCs differentiation, based on Pvec activity.
This lineage can self-renew, permitting its maintenance as a source of cardiovascular progenitor cells and constitutes a useful source for regenerative approaches.
American Psychological Association (APA)
Maltabe, Violetta A.& Barka, Eleonora& Kontonika, Marianthi& Florou, Dimitra& Kouvara-Pritsouli, Maria& Roumpi, Maria…[et al.]. 2016. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117235
Modern Language Association (MLA)
Maltabe, Violetta A.…[et al.]. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117235
American Medical Association (AMA)
Maltabe, Violetta A.& Barka, Eleonora& Kontonika, Marianthi& Florou, Dimitra& Kouvara-Pritsouli, Maria& Roumpi, Maria…[et al.]. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117235