Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes
Joint Authors
Hogerheyde, Thomas A.
Suzuki, Shuko
Walshe, Jennifer
Bray, Laura J.
Harkin, Damien G.
Richardson, Neil A.
Stephenson, S. A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-28
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Scaffolds prepared from silk fibroin derived from cocoons of the domesticated silkworm moth Bombyx mori have demonstrated potential to support the attachment and growth of human limbal epithelial (HLE) cells in vitro.
In this study, we attempted to further optimize protocols to promote the expansion of HLE cells on B.
mori silk fibroin- (BMSF-) based scaffolds.
BMSF films were initially coated with different extracellular matrix proteins and then analysed for their impact on corneal epithelial cell adhesion, cell morphology, and culture confluency.
Results showed that collagen I, collagen III, and collagen IV consistently improved HCE-T cell adherence, promoted an elongated cell morphology, and increased culture confluency.
By contrast, ECM coating had no significant effect on the performance of primary HLE cells cultured on BMSF films.
In the second part of this study, primary HLE cells were grown on BMSF films in the presence of medium (SHEM) supplemented with keratinocyte growth factor (KGF) and the Rho kinase inhibitor, Y-27632.
The results demonstrated that SHEM medium supplemented with KGF and Y-27632 dramatically increased expression of corneal differentiation markers, keratin 3 and keratin 12, whereas expression of the progenitor marker, p63, did not appear to be significantly influenced by the choice of culture medium.
American Psychological Association (APA)
Hogerheyde, Thomas A.& Suzuki, Shuko& Walshe, Jennifer& Bray, Laura J.& Stephenson, S. A.& Harkin, Damien G.…[et al.]. 2016. Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117242
Modern Language Association (MLA)
Hogerheyde, Thomas A.…[et al.]. Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117242
American Medical Association (AMA)
Hogerheyde, Thomas A.& Suzuki, Shuko& Walshe, Jennifer& Bray, Laura J.& Stephenson, S. A.& Harkin, Damien G.…[et al.]. Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117242
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117242