Hepatic Stellate Cell-Derived Microvesicles Prevent Hepatocytes from Injury Induced by APAPH2O2
Joint Authors
Miao, Huilai
Dai, Bingyan
Pan, Qunwen
Liao, Xiaorong
Huang, Renwei
Wang, Yan
Liang, Yaolong
Li, Mingyi
Ma, Xiaotang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-28
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Hepatic stellate cells (HSCs), previously described for liver-specific mesenchymal stem cells (MSCs), appear to contribute to liver regeneration.
Microvesicles (MVs) are nanoscale membrane fragments, which can regulate target cell function by transferring contents from their parent cells.
The aim of this study was to investigate the effect of HSC-derived MVs on xenobiotic-induced liver injury.
Rat and human hepatocytes, BRL-3A and HL-7702, were used to build hepatocytes injury models by n-acetyl-p-aminophenol n-(APAP) or H2O2 treatment.
MVs were prepared from human and rat HSCs, LX-2, and HST-T6 and, respectively, added to injured BRL-3A and HL-7702 hepatocytes.
MTT assay was utilized to determine cell proliferation.
Cell apoptosis was analyzed by flow cytometry and hoechst33258 staining.
Western blot was used for analyzing the expression of activated caspase-3.
Liver injury indicators, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in culture medium were also assessed.
Results showed that (1) HSC-MVs derived from LX-2 and HST-T6 were positive to CD90 and annexin V surface markers; (2) HSC-MVs dose-dependently improved the viability of hepatocytes in both injury models; (3) HSC-MVs dose-dependently inhibited the APAP/H2O2 induced hepatocytes apoptosis and activated caspase-3 expression and leakage of LDH, ALT, and AST.
Our results demonstrate that HSC-derived MVs protect hepatocytes from toxicant-induced injury.
American Psychological Association (APA)
Huang, Renwei& Pan, Qunwen& Ma, Xiaotang& Wang, Yan& Liang, Yaolong& Dai, Bingyan…[et al.]. 2016. Hepatic Stellate Cell-Derived Microvesicles Prevent Hepatocytes from Injury Induced by APAPH2O2. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117251
Modern Language Association (MLA)
Huang, Renwei…[et al.]. Hepatic Stellate Cell-Derived Microvesicles Prevent Hepatocytes from Injury Induced by APAPH2O2. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1117251
American Medical Association (AMA)
Huang, Renwei& Pan, Qunwen& Ma, Xiaotang& Wang, Yan& Liang, Yaolong& Dai, Bingyan…[et al.]. Hepatic Stellate Cell-Derived Microvesicles Prevent Hepatocytes from Injury Induced by APAPH2O2. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117251