iPSC-Derived Retinal Pigment Epithelium Allografts Do Not Elicit Detrimental Effects in Rats: A Follow-Up Study
Joint Authors
Westenskow, Peter D.
Bucher, Felicitas
Bravo, Stephen
Feitelberg, Daniel
Paris, Liliana P.
Aguilar, Edith
Lin, Jonathan H.
Friedlander, Martin
Kurihara, Toshihide
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Phototransduction is accomplished in the retina by photoreceptor neurons and retinal pigment epithelium (RPE) cells.
Photoreceptors rely heavily on the RPE, and death or dysfunction of RPE is characteristic of age-related macular degeneration (AMD), a very common neurodegenerative disease for which no cure exists.
RPE replacement is a promising therapeutic intervention for AMD, and large numbers of RPE cells can be generated from pluripotent stem cells.
However, questions persist regarding iPSC-derived RPE (iPS-RPE) viability, immunogenicity, and tumorigenesis potential.
We showed previously that iPS-RPE prevent photoreceptor atrophy in dystrophic rats up until 24 weeks after implantation.
In this follow-up study, we longitudinally monitored the same implanted iPS-RPE, in the same animals.
We observed no gross abnormalities in the eyes, livers, spleens, brains, and blood in aging rats with iPSC-RPE grafts.
iPS-RPE cells that integrated into the subretinal space outlived the photoreceptors and survived for as long as 2 1/2 years while nonintegrating RPE cells were ingested by host macrophages.
Both populations could be distinguished using immunohistochemistry and electron microscopy.
iPSC-RPE could be isolated from the grafts and maintained in culture; these cells also phagocytosed isolated photoreceptor outer segments.
We conclude that iPS-RPE grafts remain viable and do not induce any obvious associated pathological changes.
American Psychological Association (APA)
Westenskow, Peter D.& Bucher, Felicitas& Bravo, Stephen& Kurihara, Toshihide& Feitelberg, Daniel& Paris, Liliana P.…[et al.]. 2016. iPSC-Derived Retinal Pigment Epithelium Allografts Do Not Elicit Detrimental Effects in Rats: A Follow-Up Study. Stem Cells International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117273
Modern Language Association (MLA)
Westenskow, Peter D.…[et al.]. iPSC-Derived Retinal Pigment Epithelium Allografts Do Not Elicit Detrimental Effects in Rats: A Follow-Up Study. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1117273
American Medical Association (AMA)
Westenskow, Peter D.& Bucher, Felicitas& Bravo, Stephen& Kurihara, Toshihide& Feitelberg, Daniel& Paris, Liliana P.…[et al.]. iPSC-Derived Retinal Pigment Epithelium Allografts Do Not Elicit Detrimental Effects in Rats: A Follow-Up Study. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117273