Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells
Joint Authors
Conrad, Sabine
Skutella, Thomas
Hennenlotter, Jörg
Sievert, Karl D.
Azizi, Hossein
Hatami, Maryam
Kubista, Mikael
Bonin, Michael
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-08
Country of Publication
Egypt
No. of Pages
17
Abstract EN
The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia.
Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs.
Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency.
The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profile in vitro, related but not identical to true pluripotent stem cells.
Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers.
American Psychological Association (APA)
Conrad, Sabine& Azizi, Hossein& Hatami, Maryam& Kubista, Mikael& Bonin, Michael& Hennenlotter, Jörg…[et al.]. 2015. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117282
Modern Language Association (MLA)
Conrad, Sabine…[et al.]. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1117282
American Medical Association (AMA)
Conrad, Sabine& Azizi, Hossein& Hatami, Maryam& Kubista, Mikael& Bonin, Michael& Hennenlotter, Jörg…[et al.]. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117282