MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis
Joint Authors
Peroni, John F.
Scharf, Alexandra
Holmes, Shannon P.
Thoresen, Merrilee
Stumpf, Alaina
Mumaw, Jennifer
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-26
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis.
The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs.
Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 106 MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance.
Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs.
Limbs were imaged on a 1.5-tesla clinical MRI scanner postmortem before and after injection to determine the extent of tendonitis and detect SPIO MSCs.
Clusters of labeled cells were visible as signal voids in 6/6 subjects.
Coalescing regions of signal void were diffusely present in the peritendinous tissues.
Although previous reports have determined that local injury retains cells within a small radius of the site of injection, our study shows greater than expected delocalization and relatively few cells retained within collagenous tendon compared to surrounding fascia.
Further work is needed if this is a reality in vivo and to determine if directed intralesional delivery of MSCs is as critical as presently thought.
American Psychological Association (APA)
Scharf, Alexandra& Holmes, Shannon P.& Thoresen, Merrilee& Mumaw, Jennifer& Stumpf, Alaina& Peroni, John F.. 2016. MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117290
Modern Language Association (MLA)
Scharf, Alexandra…[et al.]. MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1117290
American Medical Association (AMA)
Scharf, Alexandra& Holmes, Shannon P.& Thoresen, Merrilee& Mumaw, Jennifer& Stumpf, Alaina& Peroni, John F.. MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117290