Stromal Derived Factor-1CXCR4 Axis Involved in Bone Marrow Mesenchymal Stem Cells Recruitment to Injured Liver

Joint Authors

Xiao Ling, Kuai
Peng, Li
Jian Feng, Zhang
Wei, Cao
Wei Yan, Yuan
Nan, Shao
Cheng Qi, Guan
Zhi Wei, Wang

Source

Stem Cells International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-01-12

Country of Publication

Egypt

No. of Pages

10

Abstract EN

The molecular mechanism of bone marrow mesenchymal stromal stem cells (BMSCs) mobilization and migration to the liver was poorly understood.

Stromal cell-derived factor-1 (SDF-1) participates in BMSCs homing and migration into injury organs.

We try to investigate the role of SDF-1 signaling in BMSCs migration towards injured liver.

The expression of CXCR4 in BMSCs at mRNA level and protein level was confirmed by RT-PCR, flow cytometry, and immunocytochemistry.

The SDF-1 or liver lysates induced BMSCs migration was detected by transwell inserts.

CXCR4 antagonist, AMD3100, and anti-CXCR4 antibody were used to inhibit the migration.

The Sprague-Dawley rat liver injury model was established by intraperitoneal injection of thioacetamide.

The concentration of SDF-1 increased as modeling time extended, which was determined by ELISA method.

The Dir-labeled BMSCs were injected into the liver of the rats through portal vein.

The cell migration in the liver was tracked by in vivo imaging system and the fluorescent intensity was measured.

In vivo, BMSCs migrated into injured liver which was partially blocked by AMD3100 or anti-CXCR4 antibody.

Taken together, the results demonstrated that the migration of BMSCs was regulated by SDF-1/CXCR4 signaling which involved in BMSCs recruitment to injured liver.

American Psychological Association (APA)

Xiao Ling, Kuai& Peng, Li& Jian Feng, Zhang& Wei, Cao& Wei Yan, Yuan& Nan, Shao…[et al.]. 2016. Stromal Derived Factor-1CXCR4 Axis Involved in Bone Marrow Mesenchymal Stem Cells Recruitment to Injured Liver. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117341

Modern Language Association (MLA)

Xiao Ling, Kuai…[et al.]. Stromal Derived Factor-1CXCR4 Axis Involved in Bone Marrow Mesenchymal Stem Cells Recruitment to Injured Liver. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1117341

American Medical Association (AMA)

Xiao Ling, Kuai& Peng, Li& Jian Feng, Zhang& Wei, Cao& Wei Yan, Yuan& Nan, Shao…[et al.]. Stromal Derived Factor-1CXCR4 Axis Involved in Bone Marrow Mesenchymal Stem Cells Recruitment to Injured Liver. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117341

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1117341