Comprehensive Screening of Cell Surface Markers Expressed by Adult-Derived Human Liver StemProgenitor Cells Harvested at Passage 5: Potential Implications for Engraftment
Joint Authors
Najimi, Mustapha
Sokal, Etienne
Dollet, Pierre-Edouard
Ravau, Joachim
André, Floriane
Lombard, Catherine
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-13
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Mesenchymal stromal cells (MSCs) are known to have potential therapeutic benefits for a number of diseases.
However, many studies report low engraftment levels, regardless of the target organ.
One possible explanation could be that MSCs do not express the necessary receptors for engraftment.
Indeed, MSCs appear to use a similar mechanism to leukocytes to engraft into injured organs, relying on various receptors for rolling, firm adhesion, and transmigration.
In this study, we conducted an extensive surface molecule screening of adult-derived human liver stem/progenitor cells (ADHLSC) in an attempt to shed some light on this subject.
We observed that ADHLSCs lack expression of most of the costimulatory molecules tested.
Furthermore, study of the adhesion molecule profile of ADHLSCs revealed that they do not express selectin ligands or LFA-1 which are, respectively, involved in the rolling process and the firm adhesion.
In addition, ADHLSCs slightly express VLA-4 and lose expression of CXCR4 altogether on their surface during culture expansion.
However, ADHLSCs express all the integrin couples and matrix metalloproteinases needed to bind and integrate the extracellular matrix once the endothelial barrier is crossed.
Collectively, these results suggest that binding to the endothelium may be the critical weak point in the engraftment process.
American Psychological Association (APA)
Dollet, Pierre-Edouard& Ravau, Joachim& André, Floriane& Najimi, Mustapha& Sokal, Etienne& Lombard, Catherine. 2016. Comprehensive Screening of Cell Surface Markers Expressed by Adult-Derived Human Liver StemProgenitor Cells Harvested at Passage 5: Potential Implications for Engraftment. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117399
Modern Language Association (MLA)
Dollet, Pierre-Edouard…[et al.]. Comprehensive Screening of Cell Surface Markers Expressed by Adult-Derived Human Liver StemProgenitor Cells Harvested at Passage 5: Potential Implications for Engraftment. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1117399
American Medical Association (AMA)
Dollet, Pierre-Edouard& Ravau, Joachim& André, Floriane& Najimi, Mustapha& Sokal, Etienne& Lombard, Catherine. Comprehensive Screening of Cell Surface Markers Expressed by Adult-Derived Human Liver StemProgenitor Cells Harvested at Passage 5: Potential Implications for Engraftment. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1117399
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117399