![](/images/graphics-bg.png)
Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells
Joint Authors
Glassmann, Alexander
Carrillo Garcia, Carmen
Janzen, Viktor
Kraus, Dominik
Veit, Nadine
Winter, Jochen
Probstmeier, Rainer
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner.
This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis.
Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state.
Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from.
Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis.
American Psychological Association (APA)
Glassmann, Alexander& Carrillo Garcia, Carmen& Janzen, Viktor& Kraus, Dominik& Veit, Nadine& Winter, Jochen…[et al.]. 2018. Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells. Analytical Cellular Pathology،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1117430
Modern Language Association (MLA)
Glassmann, Alexander…[et al.]. Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells. Analytical Cellular Pathology No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1117430
American Medical Association (AMA)
Glassmann, Alexander& Carrillo Garcia, Carmen& Janzen, Viktor& Kraus, Dominik& Veit, Nadine& Winter, Jochen…[et al.]. Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells. Analytical Cellular Pathology. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1117430
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117430