Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells
Joint Authors
Wu, Qiong
Zhan, Jinxi
Li, Yun
Wang, Xiaoxia
Xu, Lu
Yu, Juan
Pu, Shiming
Zhou, Zuping
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-19
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Cardiac resident stem/progenitor cells (CSC/CPCs) are critical to the cellular and functional integrity of the heart because they maintain myocardial cell homeostasis.
Several populations of CSC/CPCs have been identified based on expression of different stem cell-associated antigens.
Sca-1+ cells in the cardiac tissue may be the most common CSC/CPCs.
However, they are a heterogeneous cell population and, in transplants, clinicians might transplant more endothelial cells, cardiomyocytes, or other cells than stem cells.
The purposes of this study were to (1) isolate CSC/CPCs with Lin−CD45−Sca-1+CD31− and Lin−CD45−Sca-1+CD31+ surface antigens using flow-activated cell sorting; (2) investigate their differentiation potential; and (3) determine the molecular basis for differences in stemness characteristics between cell subtypes.
The results indicated that mouse heart-derived Sca-1+CD31− cells were multipotent and retained the ability to differentiate into different cardiac cell lineages, but Sca-1+CD31+ cells did not.
Integrated analysis of microRNA and mRNA expression indicated that 20 microRNAs and 49 mRNAs were inversely associated with Sca-1+CD31− and Sca-1+CD31+ subtype stemness characteristics.
In particular, mmu-miR-322-5p had more targeted and inversely associated genes and transcription factors and might have higher potential for CSC/CPCs differentiation.
American Psychological Association (APA)
Wu, Qiong& Zhan, Jinxi& Li, Yun& Wang, Xiaoxia& Xu, Lu& Yu, Juan…[et al.]. 2016. Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117436
Modern Language Association (MLA)
Wu, Qiong…[et al.]. Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1117436
American Medical Association (AMA)
Wu, Qiong& Zhan, Jinxi& Li, Yun& Wang, Xiaoxia& Xu, Lu& Yu, Juan…[et al.]. Differentiation-Associated MicroRNA Alterations in Mouse Heart-Derived Sca-1+CD31− and Sca-1+CD31+ Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1117436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117436