Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155
Joint Authors
Ruan, Yang
Li, Hong
Pu, Lianmei
Shen, Tao
Jin, Zening
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
To investigate the function of Tremella fuciformis polysaccharides (TFPS) in LPS-induced inflammation and oxidative stress of macrophages.
Methods.
RAW264.7 cells were pretreated with TFPS and then stimulated with 0.1 μg/ml LPS.
NFκB, Akt, p38MAPK, MCP-1, and SOD-1 were analyzed by Western blotting.
Cell viability was measured using MTT assays.
Reactive oxygen species (ROS) production, real-time PCR, ELISA, and immunofluorescence staining were performed on RAW264.7 cells that were treated with LPS and/or TFPS to investigate the anti-inflammatory effect of TFPS.
Results.
LPS induced inflammation and ROS production and promoted the secretion of cytokines such as TNF-α and IL-6.
LPS also enhanced the nuclear translocation of NFκB, which promoted inflammation by oxidative stress.
However, pretreatment with TFPS profoundly inhibited the activation of Akt, p38MAPK, and NFκB and attenuated the expression of MCP-1 in macrophages.
Meanwhile, TFPS also decreased cytokine and ROS levels and attenuated cell inflammation after treatment with LPS.
Moreover, miR-155, one of the key small RNAs which regulate NFκB and inflammation in macrophages, was significantly downregulated.
Conclusion.
TFPS inhibits LPS-induced oxidative stress and inflammation by inhibiting miR-155 expression and NFκB activation in macrophages, which suggests that TFPS may be a potential reagent for inhibiting the development of inflammation.
American Psychological Association (APA)
Ruan, Yang& Li, Hong& Pu, Lianmei& Shen, Tao& Jin, Zening. 2018. Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155. Analytical Cellular Pathology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1117468
Modern Language Association (MLA)
Ruan, Yang…[et al.]. Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155. Analytical Cellular Pathology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1117468
American Medical Association (AMA)
Ruan, Yang& Li, Hong& Pu, Lianmei& Shen, Tao& Jin, Zening. Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155. Analytical Cellular Pathology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1117468
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117468