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Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
Author
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-16
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination.
In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms.
We also studied the correlation of Hb A1c with age.
Hemoglobin analysis was carried out by high performance liquid chromatography.
Spearman’s rank correlation was used to study correlation between A1c levels and age.
Mann-Whitney U test was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits.
A total of 431 patients were studied.
There was positive correlation with age in patients with normal chromatograms only.
No correlation was seen in Hb E trait or beta thalassemia trait.
No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen.
There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography.
This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.
American Psychological Association (APA)
Chandrashekar, Vani. 2016. Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies. Scientifica،Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1117584
Modern Language Association (MLA)
Chandrashekar, Vani. Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies. Scientifica No. 2016 (2016), pp.1-4.
https://search.emarefa.net/detail/BIM-1117584
American Medical Association (AMA)
Chandrashekar, Vani. Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1117584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117584