The Effect of Protein FAM172A on Proliferation in HepG2 Cells and Investigation of the Possible Molecular Mechanism
Joint Authors
Zhao, Hong
Wang, Yujie
Liu, Yufeng
Hao, Xiaohua
Wei, Hongshan
Xie, Wen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
In our previous study, we found that the FAM172A recombinant protein could promote proliferation of L02 cells.
However, the underlying mechanisms are still unknown.
The present study was aimed at investigating the effect of FAM172A on proliferation of HepG2 cells and exploring the possible molecular mechanisms and its role in hepatocellular carcinoma (HCC).
Methods.
Cell proliferation was measured by MTT assay.
Western blot test was carried out to investigate the mechanism.
Rabbit antibodies against FAM172A and membrane proteins isolated from lysate of HepG2 cell were coprecipitated and the resultant precipitates were analyzed by mass spectrum.
Results.
The MTT assay showed that recombinant protein FAM172A isoform 1 (FAM172A-1) could induce HepG2 cell proliferation at the concentration of 10-100 ng/mL, while protein FAM172A isoform 3 (FAM172A-3) was at the concentration of 80-100 ng/mL.
Western blot demonstrated that both FAM172A-1 and FAM172A-3 could activate the mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) pathway and the phosphatidylinositol 3-kinase/threonine-protein kinase (PI3K/Akt) pathway.
Mass spectrum analysis suggested that there were some membrane proteins interacting with FAM172A.
Several candidate interacting proteins might mediate proliferation signals induced by FAM172A recombinant protein, including seven membrane proteins.
Conclusion.
In conclusion, FAM172A recombinant protein could induce proliferation of HepG2 cells, in which the MAPK/ERK and PI3K/Akt signaling pathways might be involved.
The role of FAM172A in HepG2 cell proliferation also indicated its possible involvement in HCC.
The receptor of FAM172A on cells still needs to be exploited.
American Psychological Association (APA)
Zhao, Hong& Wang, Yujie& Liu, Yufeng& Hao, Xiaohua& Wei, Hongshan& Xie, Wen. 2019. The Effect of Protein FAM172A on Proliferation in HepG2 Cells and Investigation of the Possible Molecular Mechanism. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1117860
Modern Language Association (MLA)
Zhao, Hong…[et al.]. The Effect of Protein FAM172A on Proliferation in HepG2 Cells and Investigation of the Possible Molecular Mechanism. Analytical Cellular Pathology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1117860
American Medical Association (AMA)
Zhao, Hong& Wang, Yujie& Liu, Yufeng& Hao, Xiaohua& Wei, Hongshan& Xie, Wen. The Effect of Protein FAM172A on Proliferation in HepG2 Cells and Investigation of the Possible Molecular Mechanism. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1117860
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117860