Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism
Joint Authors
Zeng, Hua-jin
Yang, Ran
You, Jing
Qu, Ling-bo
Sun, Yan-jun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The inhibitory effect of liquiritigenin on hyaluronidase and its binding mechanism were investigated systematically by UV-vis absorption, fluorescence, and molecular modeling approaches.
These results indicated that liquiritigenin could interact with hyaluronidase to form a liquiritigenin-hyaluronidase complex.
The binding constant, number of binding sites, and thermodynamic parameters were calculated, which indicated that liquiritigenin could spontaneously bind with hyaluronidase mainly through electrostatic and hydrophobic interactions with one binding site.
Synchronous fluorescence, three-dimensional fluorescence, and molecular docking results revealed that liquiritigenin bound directly to the enzyme cavity site and this binding influenced the microenvironment of the hyaluronidase activity site, resulting in reduced hyaluronidase activity.
The present study provides useful information for clinical applications of liquiritigenin as a hyaluronidase inhibitor.
American Psychological Association (APA)
Zeng, Hua-jin& Yang, Ran& You, Jing& Qu, Ling-bo& Sun, Yan-jun. 2016. Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism. Scientifica،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117973
Modern Language Association (MLA)
Zeng, Hua-jin…[et al.]. Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism. Scientifica No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1117973
American Medical Association (AMA)
Zeng, Hua-jin& Yang, Ran& You, Jing& Qu, Ling-bo& Sun, Yan-jun. Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1117973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117973