Molecular and Growth-Based Drug Susceptibility Testing of Mycobacterium tuberculosis Complex for Ethambutol Resistance in the United States
Joint Authors
Yakrus, Mitchell A.
Metchock, Beverly
Starks, Angela M.
Driscoll, Jeffrey
McAlister, Allison
Sikes, David
Hartline, Denise
Source
Tuberculosis Research and Treatment
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-08
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Ethambutol (EMB) is used as a part of drug regimens for treatment of tuberculosis (TB).
Susceptibility of Mycobacterium tuberculosis complex (MTBC) isolates to EMB can be discerned by DNA sequencing to detect mutations in the embB gene associated with resistance.
US Public Health Laboratories (PHL) primarily use growth-based drug susceptibility test (DST) methods to determine EMB resistance.
The Centers for Disease Control and Prevention (CDC) provides a service for molecular detection of drug resistance (MDDR) by DNA sequencing and concurrent growth-based DST using agar proportion.
PHL and CDC test results were compared for 211 MTBC samples submitted to CDC from September 2009 through February 2011.
Concordance between growth-based DST results from PHL and CDC was 88.2%.
A growth-based comparison of 39 samples, where an embB mutation associated with EMB resistance was detected, revealed a higher percentage of EMB resistance by CDC (84.6%) than by PHL (59.0%) which was significant ( P value = 0.002).
Discordance between all growth-based test results from PHL and CDC was also significant ( P value = 0.003).
Most discordance was linked to false susceptibility using the BACTEC™ MGIT™ 960 (MGIT) growth-based system.
Our analysis supports coalescing growth-based and molecular results for an informed interpretation of potential EMB resistance.
American Psychological Association (APA)
Yakrus, Mitchell A.& Driscoll, Jeffrey& McAlister, Allison& Sikes, David& Hartline, Denise& Metchock, Beverly…[et al.]. 2016. Molecular and Growth-Based Drug Susceptibility Testing of Mycobacterium tuberculosis Complex for Ethambutol Resistance in the United States. Tuberculosis Research and Treatment،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1120254
Modern Language Association (MLA)
Yakrus, Mitchell A.…[et al.]. Molecular and Growth-Based Drug Susceptibility Testing of Mycobacterium tuberculosis Complex for Ethambutol Resistance in the United States. Tuberculosis Research and Treatment No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1120254
American Medical Association (AMA)
Yakrus, Mitchell A.& Driscoll, Jeffrey& McAlister, Allison& Sikes, David& Hartline, Denise& Metchock, Beverly…[et al.]. Molecular and Growth-Based Drug Susceptibility Testing of Mycobacterium tuberculosis Complex for Ethambutol Resistance in the United States. Tuberculosis Research and Treatment. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1120254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120254