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Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India
Joint Authors
Mir, Abdul Rouf
Dar, Firdous Ahmad
Sekhar, M.
Bashir, Yasir
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E.
coli obtained from patients from Chennai, India.
Isolation and identification of pathogens were done on MacConkey agar.
Antimicrobial sensitivity testing was done by disc diffusion test.
The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR).
Out of 98 isolates, 71 (72.45%) isolates were identified as E.
coli and the remaining 27 (27.55%) as other bacteria.
Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E.
coli.
PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA.
The results point towards the novel mechanism of drug resistance in E.
coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs.
This could be an alarm for drug prescription to UTI patients.
American Psychological Association (APA)
Mir, Abdul Rouf& Bashir, Yasir& Dar, Firdous Ahmad& Sekhar, M.. 2016. Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India. The Scientific World Journal،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1120324
Modern Language Association (MLA)
Mir, Abdul Rouf…[et al.]. Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India. The Scientific World Journal No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1120324
American Medical Association (AMA)
Mir, Abdul Rouf& Bashir, Yasir& Dar, Firdous Ahmad& Sekhar, M.. Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India. The Scientific World Journal. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1120324
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120324