Origanum majorana Essential Oil Lacks Mutagenic Activity in the SalmonellaMicrosome and Micronucleus Assays
Joint Authors
Machado, Miriana
Pêgas Henriques, João Antonio
Guecheva, Temenouga Nikolova
Dantas, Andrea dos Santos
Klein-Júnior, Luiz Carlos
Santos, Luciana D. dos
Zanette, Régis A.
Mello, Fernanda B. de
Mello, João Roberto Braga de
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The present study aimed to investigate the in vitro mutagenic activity of Origanum majorana essential oil.
The most abundant compounds identified by GC-MS were γ-terpinene (25.73%), α-terpinene (17.35%), terpinen-4-ol (17.24%), and sabinene (10.8%).
Mutagenicity was evaluated by the Salmonella/microsome test using the preincubation procedure on TA98, TA97a, TA100, TA102, and TA1535 Salmonella typhimurium strains, in the absence or in the presence of metabolic activation.
Cytotoxicity was detected at concentrations higher than 0.04 μL/plate in the absence of S9 mix and higher than 0.08 μL/plate in the presence of S9 mix and no gene mutation increase was observed.
For the in vitro mammalian cell micronucleus test, V79 Chinese hamster lung fibroblasts were used.
Cytotoxicity was only observed at concentrations higher than or equal to 0.05 μg/mL.
Moreover, when tested in noncytotoxic concentrations, O.
majorana essential oil was not able to induce chromosome mutation.
The results from this study therefore suggest that O.
majorana essential oil is not mutagenic at the concentrations tested in the Salmonella/microsome and micronucleus assays.
American Psychological Association (APA)
Dantas, Andrea dos Santos& Klein-Júnior, Luiz Carlos& Machado, Miriana& Guecheva, Temenouga Nikolova& Santos, Luciana D. dos& Zanette, Régis A.…[et al.]. 2016. Origanum majorana Essential Oil Lacks Mutagenic Activity in the SalmonellaMicrosome and Micronucleus Assays. The Scientific World Journal،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1120407
Modern Language Association (MLA)
Dantas, Andrea dos Santos…[et al.]. Origanum majorana Essential Oil Lacks Mutagenic Activity in the SalmonellaMicrosome and Micronucleus Assays. The Scientific World Journal No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1120407
American Medical Association (AMA)
Dantas, Andrea dos Santos& Klein-Júnior, Luiz Carlos& Machado, Miriana& Guecheva, Temenouga Nikolova& Santos, Luciana D. dos& Zanette, Régis A.…[et al.]. Origanum majorana Essential Oil Lacks Mutagenic Activity in the SalmonellaMicrosome and Micronucleus Assays. The Scientific World Journal. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1120407
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120407